Fig. 1

PTP4A1 is overexpressed in SSc DFs. a Mean ± SEM of PTP4A1 mRNA expression measured in DF lines from 13 healthy donors or patients with SSc. b Mean ± SEM of densitometric scan expression plus representative immunoblotting for PTP4A1/2 (upper bands) normalized to αtubulin (lower bands) in five healthy and four SSc DF lines. Images are from distinct membranes. c PTP4A1/2 (green signal) and αSMA (red signal) IF in skin sections from healthy or SSc diffuse early, diffuse late, and limited donors with Hoechst nuclear staining (blue signal). PTP4A1/2-αSMA co-localization signal appears orange in the images. Right panels show magnified area from yellow squares in left panels. d, e Mean ± SEM of PTP4A1/2 fluorescence densitometry signal in αSMA positive skin cells from two cohorts: the first one d including six healthy or five SSc diffuse early, six diffuse late and six limited donors; the second one e including five healthy or five SSc early and five late donors. Mann–Whitney test. Scale bars, 50 μm