Fig. 3
From: PTP4A1 promotes TGFβ signaling and fibrosis in systemic sclerosis
PTP4A1 and PTP4A2 silencing inhibits TGFβ signalings. a, b Mean ± SEM of COL1A2 and ACTA2 mRNA expression measured in six different NHDF lines treated with PTP4A1 ASO, stimulated with TGFβ1 and normalized to the same lines treated with control ASO. c Mean ± SEM of collagen concentration measured in three different NHDF lines treated with control or PTP4A1 ASO and stimulated with TGFβ1. d, e Mean ± SEM of COL1A2 and ACTA2 mRNA expression measured in three different NHDF lines treated with PTP4A2 ASO, stimulated with TGFβ1 and normalized to the same lines treated with control ASO. f Mean ± SEM of SMAD3 mRNA expression measured in three different NHDF lines treated with PTP4A2 ASO and normalized to the same lines treated with control ASO. g Mean ± SEM of densitometric scan expression plus representative immunoblotting for SMAD3 (upper bands) normalized to GAPDH (lower bands) in three different NHDF lines treated with control ASO or PTP4A2 ASO. Images are from different lanes on the same membrane. Wilcoxon test (a, b), Welch’s t-test (c), or Paired t-test (d, e)
