Fig. 1
Indeterminate phenotype of the crc knu double mutant is caused by WUS activity. a–d The floral meristem activity marker WUS mRNA in longitudinal sections of wild-type (a) crc-1 (b) knu-1 (c), and crc-1 knu-1 (d) floral buds at developmental stage 6. e–h Flower phenotypes in wus-1 (e), wus-1 crc-1 (f), wus-1 knu-1 (g), and wus-1 crc-1 knu-1 (h). i, j Morphology of crc-1 knu-1 (i) and wus-1 −/+;crc-1 knu-1 (j) fruits. Top: close-up views of fruit tips. Bottom: shapes of whole fruits. Arrowheads indicate stigma structures. Two stigma-like structures were observed in the wus-1 −/+ crc-1 knu-1 mutant due to carpel separation. Images are shown at the same magnification. k Quantification of mutant phenotype. p-values were calculated by χ 2 test (n = 30). l, m Longitudinal sections of crc-1 knu-1 (l) and wus-1 −/+ crc-1 knu-1 (m) fruits. Asterisks indicate ectopic carpels. n, o Scanning electron micrographs of fruits with reiterations of floral organs in crc-1 knu-1 (n) and wus-1 −/+ crc-1 knu-1 (o) fruits. Bars = 50 μm in a–d; 5 mm in e–h; 1 cm in i, j bottom; 500 μm in l, m; and 1 mm in n, o
