Fig. 2
Indeterminate phenotype of the crc knu double mutant is rescued by auxin transport inhibitor treatment. a GO terms enriched among the 210 upregulated gene in crc knu. For a list of these genes, see Supplementary Data 1 and 2. FDR cutoff of <0.03% was implemented. The −log10 adjusted p-values of all significant GO terms are shown. b List of the six genes with likely roles in metabolic process and meristem activity. Among the six genes, EMB3105 and TRN2 play a role in regulating ‘auxin polar transport’, as highlighted in red. c, d Morphology of mock-treated crc-1 knu-1 (c) and NPA-treated crc-1 knu-1 (d) fruits. Above: close-up views of fruit tips. Below: shapes of whole fruits. Arrowhead indicates stigma structures. e Quantification of mutant phenotype. p-values were calculated by χ 2 test (n = 30). f, g Longitudinal section of mock-treated crc-1 knu-1 (f) and NPA-treated crc-1 knu-1 (g) fruits. Asterisks indicate carpels. h, i Scanning electron micrograph of crc-1 knu-1 fruits. h Mock-treated crc-1 knu-1 fruits with several reiterations of floral organs. i NPA-treated crc-1 knu-1 fruits with reduced reiterations. j, k pPIN1::PIN1-GFP expression in wild-type (j) and crc-1 (k) flowers. l, m In situ hybridization showing the distribution of the adaxial fate marker ARGONAUTE10 (AGO10) in longitudinal sections of wild-type (l), and crc-1 (m) floral buds at developmental stage 6. n–r DR5rev::3xVENUS expression in wild-type (n), mock-treated crc-1 (o), NPA-treated crc-1 (p), mock-treated crc-1 knu-1 (q), and NPA-treated crc-1 knu-1 (r) floral buds at developmental stage 6. Insets are closer inspection of floral buds. Arrowheads indicate medial region of gynoecium. Bars = 1 cm in c, d bottom; 500 µm in f, g; 1 mm in h, i; 50 μm in j, k and n–o; and 25 μm in l, m
