Fig. 3
From: Structure of human lysosomal acid α-glucosidase–a guide for the treatment of Pompe disease
Ligand binding to rhGAA. DNJ (a), NHE-DNJ (c) and acarbose (e) colored in orange bound to rhGAA, color-coded as in Fig. 2c, overlapped onto unbound rhGAA (grey) in (a) and (c). Hydrogen bonding interactions are represented as dashed lines. In e, substrate-binding subsites are numbered and residues of a symmetry-related molecule are depicted in white sticks. Unbiased F o –F c difference electron density maps, calculated before incorporation of the ligands into the models and contored at 3.0 σ, are shown in b, d and f
