Fig. 1
From: Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions
GFP-RAB6-positive vesicles exit the Golgi at fission hotspots. a HeLa cells stably expressing GFP-RAB6 were imaged every second for 1 min (Supplementary Movie 1). Orange circles show the location of five fission hotspots. Details of each fission event for the five regions of hotspots are displayed. In each case, sequences of images are displayed at the top and a scheme of the events at the bottom. The Golgi region is highlighted by a white line. Vesicles exiting the Golgi are drawn and painted either yellow-green or orange-red. A sum of the events is shown at the end of each sequence. b Cells were treated for 45 min with para-nitro-blebbistatin. Then, para-nitro-blebbistatin was washed out. After recovery of normal GFP-RAB6 vesicles trafficking, the cells were imaged every second for 60 s (Supplementary Movie 3). A scheme of the images is displayed at the bottom. The Golgi regions are highlighted by a white line. Tubes are drawn in yellow. Regions where the GFP-RAB6-positive fission hotspots are found are drawn in red. Bars: 10 µm
