Fig. 2
From: Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs
NMR spectra confirm G-quadruplex formation. a Imino region of the1H NMR spectra of 5ʹtiRNAAla. Spectra were recorded at 25 °C in 90% H2O/10% D2O(v/v) in the presence of 50 mM KCl, 10 mM potassium phosphate, 0.1 mM EDTA, pH 6.8 (top panel), 150 mM NaCl, 10 mM sodium phosphate, 0.1 mM EDTA, pH 6.8 (middle panel), and 150 mM LiCl, 10 mM Tris-HCl, 0.1 mM EDTA, pH 6.8 (bottom panel). b Temperature dependence of the imino region of the 1H NMR spectra of 5ʹtiRNAAla. Spectra were recorded in 90% H2O/10% D2O(v/v) in the presence of 50 mM KCl, 10 mM potassium phosphate, 0.1 mM EDTA, pH 6.8 (left panels), 150 mM NaCl, 10 mM sodium phosphate, 0.1 mM EDTA, pH 6.8 (middle panels), and 150 mM LiCl, 10 mM Tris-HCl, 0.1 mM EDTA, pH 6.8 (right panels). c Imino region of 5ʹtiRNAAla in 90% H2O/10% D2O (lower panels) and after 12 h in D2O (upper panels) at 45 °C in the presence of 150 mM NaCl, 10 mM sodium phosphate, 0.1 mM EDTA, pH 6.8 (left panels) and 50 mM KCl, 10 mM potassium phosphate and 0.1 mM EDTA, pH 6.8 (right panels). d CD spectra of 5ʹtiRNAAla at 25 °C. The spectra were recorded in the presence of 50 mM KCl, 10 mM potassium phosphate and 0.1 mM EDTA, pH 6.8 (black), 150 mM NaCl, 10 mM sodium phosphate, 0.1 mM EDTA, pH 6.8 (red) and 150 mM LiCl, 10 mM Tris-HCl, 0.1 mM EDTA, pH 6.8 (blue)
