Fig. 6
From: Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs
tRNA fragments from Arabadopsis thaliana form RG4 which are required for their activity. a Secondary structures of three 5ʹtiRNA species previously identified in salt stressed A. thaliana harboring putative 5ʹTOG motifs. b Analysis of A. thaliana tiRNAs on 15% denaturing acrylamide gel post-stained with either SYBR Gold or NMM to detect total RNA or RG4 structures, respectively. AtRNA90 forms a strong RG4 positive band which runs in a similar manner to 5ʹtiRNAAla. Upon equilibration in LiCl, the more slowly migrating RG4 positive band disappears. c In vitro translation in rabbit reticulocyte lysates using salt equilibrated or untreated (NT) A. thaliana RNAs. Depletion of RG4 structures in AtRNA90 inhibits its ability to repress translation. However, other active A. thaliana tiRNAs repress translations despite ionic equilibration suggesting they inhibit translation via an alternative mechanism
