Fig. 1

Axn mutant clones cause supernumerary polar cells. a Drawing of a Drosophila ovariole in the sagittal view. Dashed arrow indicates the border cell migration path. b Sagittal view of stage 10 egg chambers with control (left panel) or FRT82B, Axn ¹⁵¹¹ mosaic (right panel) border cell clusters (dashed boxes). Scale bar, 50 μm. c 3D projection view of border cell clusters containing FRT82B control or FRT82B, Axn ¹⁵¹¹ mosaic clones. Homozygous mutant cells are RFP₋negative (RFP⁻). Polar cells are identified by absence of Eya expression (dotted circles). Scale bar, 10 μm. d Quantification of all border cell clusters in stage 9/10 egg chambers, regardless of whether they have clones or not, in FRT82B control or FRT82B, Axn ¹⁵¹¹, 4-5 days after clone induction. Data from n = 284 egg chambers for control, 222 for Axn ⁻. Each dot represents one border cell cluster. Lines show the median with interquartile range. e Border cell cluster in FRT42D control or FRT42D, ptc ^S2 mutant clones. Homozygous mutant cells are RFP⁻. Polar cells are Eya⁻ (dotted circles). Scale bar, 10 μm