Fig. 7

Notch is permissive but not instructive for differentiation of multiple cell types. a NRE-GFP pattern from the germarium to stage 4 egg chambers. Arrows point to Notch activity in the cap cells and region 2a/2b boundary cells and arrowheads point to polar cells. b Quantification of NRE-GFP intensity in cap cells, escort cells, and follicle cells along the anterior–posterior axis until stage 4. Data from n = 1533 cells in 3 ovarioles. Different colours represent different ovarioles. Data were normalized to maximum NRE-GFP intensity per sample. c NRE-GFP in germaria with NotchRNAi mutant clones (red diamonds) compared to control cells in the same germarium (blue triangles). d Quantification of NRE-GFP in germaria region 2a and 2b with mosaic clones. Data (median with interquartile range) from n = 20–66 cells from 3 germaria. Data were normalized to average NRE intensity in internal control cells. **P < 0.01, ****P < 0.0001 (Mann–Whitney test) e Eya and Cas intensity in germaria with FRT19A control (orange diamonds), NotchRNAi, or UAS-N.intra mutant clones (red diamonds) compared to control cells in the same germarium (blue triangles). *P < 0.05, **P < 0.01 (Unpaired t-test), f Quantification of Eya and Cas intensity in germarium region 2b containing FRT19A control, Notch ^55e11, NotchRNAi, fng ¹³, or UAS-N.intra mutant FSC clones. Data (median with interquartile range) from n = 17-60 cells from 4 germaria per genotype. Data were normalized to average Eya or Cas in internal control cells. Samples labelled with different letters are significantly different at P < 0.01 (Kruskal–Wallis with Dunn’s test). g 3D projection view of one half of stage 4 egg chambers with FRT82B control, NotchRNAi, UAS-N.intra, Axn ^S044230, Axn ^S044230 + NotchRNAi, or Axn ^S044230 + UAS-N.intra mosaic FSC clones (GFP⁺, dashed lines). Eya⁺ GFP⁻ cells appear in the outlined Axn ^S044230 clone due to Z stack projection. h Quantification of Eya and Cas intensity in follicle cells with FRT82B control, NotchRNAi, UAS-N.intra, Axn ^S044230, Axn ^S044230 + NotchRNAi, or Axn ^S044230 + UAS-N.intra mosaic FSC clones. Data from n = 1051–1420 cells from 4 stage 4 egg chambers per genotype. Scale bars, 20 μm