Fig. 4

PI3K/AKT inhibitor post-translationally downregulate TRF1 levels. a Quantification of total nuclear TRF1 levels in lung cancer-derived cells (CHA 9-3) treated either with DMSO, AKT inhibitor (AKTi) (10 μM), ETP47037 (10 μM), or ETP47228 (10 μM). A representative western blot image is shown below. b Quantification of total nuclear TRF1 levels in in immortalized p53 ^−/− MEFs treated either with DMSO, AKT inhibitor (AKTi) (10 μM), or ETP47037 (10 μM). A representative western blot image is shown below. c Quantification of total nuclear TRF1 levels in P110α ^lox/lox MEFs cells transduced with pBabe-GFP or with pBabe-Cre recombinase. A representative WB images is shown below. d Quantification by immunofluorescence of mean TRF1 foci intensity in CHA 9-3 cells treated either with DMSO, DMSO plus bortezomib (50 nM), ETP47037 (10 μM) plus bortezomib (50 nM), ETP47228 (10 μM) plus bortezomib (50 nM), and AKTi (10 μM) plus bortezomib (50 nM). Student’s t test was used for statistical analysis; P values are shown. Error bars represent standard error. n number of independent experiments