Fig. 5
From: Homeostatic plasticity shapes the visual system’s first synapse
RBC plasticity is independent of light-evoked rod input. a Representative ERG responses to stimuli of increasing light intensity (top row: 0.00025 cd/m2, middle row: 0.25 cd/m2, bottom row: 25 cd/m2) recorded from wild-type (left column) and Gnat1 −/− (right column) retinas. b Summary data of intensity response functions measured from b-wave amplitudes in wild-type (open circles, n = 6 mice) and Gnat1 −/− (filled circles, n = 4 mice) animals. c, d MIP of dendritic trees of individual RBCs labeled via AAVs (Grm6 S -tdTomato) or in a transgenic line (Grm6 L-tdTomato) in Gnat1 −/− (c) and Gnat1 −/− Pax6-DTA (d) mice. For visual clarity, RBCs were digitally isolated in Amira. Scale bar indicates 5 μm. e, f Maps of dendritic territories (gray shaded areas) and synapse configurations (singlets: green circles, doublets: red circles) of the cells shown in c and d. Scale bar indicates 5 μm. g, h Summary data of RBC dendritic territories in Gnat1 −/− (g, n = 13 RBCs, n = 5 mice) and Gnat1 −/− Pax6-DTA (i, n = 8 RBCs, n = 4 mice, p < 0.001) mice. i, j Population data (mean ± SEM) of the distribution of synapses configurations on RBC dendrites in Gnat1 −/− (i, n = 11 RBCs) and Gnat1 −/− Pax6-DTA (j, n = 8 RBCs, n = 4 mice, p < 0.04) retinas
