Fig. 4
From: Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations
Immune tolerance in CH103 germ line knock-in mice. a–c Bone Marrow B cell development in naive CH103 unmutated common ancestor (UCA) double knock-in (dKI) mice, compared to wild type (B6), showing anergic phenotype and reduced frequencies of mature B cells associated with the second tolerance checkpoint. a Flow histograms indicating percentages in progenitor/precursor (Pro/pre), Immature (Imm), Transitional (Trans), and Mature (Mat) subsets. Data gated on live, total (CD19+ B220+) B cells. b Graphical summary of data shown in (a) for several mice. c–e Peripheral B cell development in naive CH103 UCA dKI mice, showing reduced total B cell numbers and frequencies of follicular mature B cells. c Representative flow histograms indicating percentages of total, live gated splenic B cells in transitional (Trans), marginal zone (MZ), and mature follicular (Mat) subsets. Graphical representation of total splenic B cell numbers (d) or mature to transitional ratios (e), as a measure of developmental arrest (transitional B cell accumulation) in periphery. Circles represent individual mice, and means are denoted by black bars. *P < 0.05; **P < 0.005, two-tailed Student’s t-test. n.s. not significant
