Fig. 3
From: Bioluminescence imaging of G protein-coupled receptor activation in living mice
S1P1 activation after treatment with S1P1 agonists in live mice. a S1P1 luciferase signaling (S1P1LS) mice were intraperitoneally injected with vehicle or the S1P1 agonists FTY720 or RP-001, then subjected to BLI. b Mice were subjected to imaging prior to injection (baseline, B/L) and 1.5, 6, 24, and 48 h after injection. Representative bioluminescence images of the same three mice comparing the effects of vehicle (Veh), FTY720 (FTY), or RP-001 (RP) at the specified time points. Mice were imaged in the supine position. Red open rectangles representing ROI were positioned around cephalic, thoracic, and epigastric regions. c–e The bioluminescence was quantified by determining the total flux (photons/sec; p/sec) in the cephalic (c), thoracic (d), and epigastric (e) ROI. Data represent the mean ± SEM. n = 5 for vehicle, n = 8 for FTY720, n = 6 for RP-001. P values between vehicle and agonist-injected groups were determined by one-way ANOVA followed by Bonferroni’s multiple comparisons test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001
