Fig. 4
From: Bioluminescence imaging of G protein-coupled receptor activation in living mice
S1P1 activation during LPS-induced systemic inflammation in live mice. a S1P1 luciferase signaling (S1P1LS) mice were injected intraperitoneally with LPS to induce systemic inflammation, then subjected to BLI. b Representative bioluminescence images of the same three mice prior to LPS injection (baseline, B/L) and 2, 6, 24, 48, and 72 h after LPS injection. Mice were imaged in the supine position. Red open rectangles representing ROI were positioned around cephalic, thoracic, and epigastric regions. c The bioluminescence activity was quantified by determining the total flux (photons/sec; p/sec) in each ROI. Data represent the mean ± SEM. n = 5. P values were determined between baseline and each time point by one-way ANOVA followed by Bonferroni’s multiple comparisons test; **P ≤ 0.01, ***P ≤ 0.001
