Fig. 1
From: Diversity oriented biosynthesis via accelerated evolution of modular gene clusters
Pathway for the biosynthesis of 1 and organisation of the rapamycin PKS. The repetitive, modular nature of each PKS protein is clearly evident. The circles represent individual catalytic domains: CoL = CoA ligase like; KS = β-ketoacyl-ACP synthase; AT = acyltransferase; DH = dehydratase; ER = enoyl reductase; KR = β-ketoacyl-ACP reductase; ACP = acylcarrier protein. Filled circles represent inactive domains. hCHCA = trans-4-hydroxycyclohexanecarboxylic acid which is fed to the strain ISOM-4010 to initiate the biosynthesis of 1 and rapalogs 2–8
