Fig. 4
Biochemical detection of abnormal PrP in CNS samples of primates. a Direct detection of PrP accumulation in spinal cord samples from primates after purification in the absence (−PK) or in the presence (+PK) of proteolysis (40 µg/ml proteinase K for 10 min) detected with 3F4, Saf-37 and Saf-60 antibodies. The equivalent of the same amount of material (8 mg) was deposited in each line. Corresponding uncropped western blots are shown in Supplementary Fig. 5. b Amplification of abnormal PrP by RT-QuiC in brain (white bars) or spinal cord (black bar) primate samples. The mean fluorescence from six replicates (two independent experiments with triplicates) after 50 h of amplification is presented here. c Amplification of abnormal PrP by PMCA reaction in brain or spinal cord primate samples. Four individual replicates of each sample, including serial dilutions of positive control, were tested. For each sample at each round, the PrPres positive replicates are figured (red crosses). The blue circles figure the negative replicates after five runs. D6, R1, R4 and R15: vCJD primates; D7, R5, R6, R8, R10, R16, R17, R18, R19: myelopathic primates; CTL1, CTL2: healthy primates. Details on each primate are mentioned in Supplementary Note 1
