Fig. 4

miR-34a increases apoptosis in lung epithelial cells. a MLE12 cells were transfected with either the N.C. mimic or miR-34a mimic and exposed to hyperoxia for 48 h and subjected to Annexin V and Propidium Iodide (PI) assay. Graph showing significantly increased FACS analysis of Annexin V and PI staining positive cells in miR-34a mimic transfected group compared to its control. b Representative graph showing significantly deccreased Annexin V and PI staining positive cells in miR-34a inhibitor transfected group compared to its control in hyperoxia exposed MLE12 cells. c Western blot image showing increased Tie2 and Ang1 and decreased cleaved caspase 3 in miR-34a inhibitor transfected group compared to its control in hyperoxia exposed MLE12 cells. d Densitometric analysis showing significantly decreased ratio of cleaved caspase 3 to total caspase 3. e Densitometric analysis showing increased Tie2 (n = 1). f Densitometric analysis showing increased Ang1 (n = 1). Values are means + SEM of a minimum of four experiments, unless otherwise stated. N.C.: Negative control. *P <0.05, **P <0.01, ***P <0.001, ****P <0.0001 compared with controls, 1-way ANOVA