Fig. 9
From: Hyperoxia causes miR-34a-mediated injury via angiopoietin-1 in neonatal lungs
Improved effects of miR-34a inhibition occurs via Ang1-Tie2 signaling. a, b Representative bar graphs show improved TUNEL and morphometry in recombinant Ang1 (rAng1) treated mouse lungs. c, d Improved RV to LV and RV/(LV + IVS) ratios were noted in rAng1 treated BPD mice as compared to controls. e Activation of Erk and Akt signalling by rAng1 in miR-34a inhibitor or scrambled control transfected MLE12 cells as analyzed by western blot of phosho-Erk1/2, total Erk1/2 protein, phospho-Akt and total Akt protein, shown at the indicated time points. Ang1: angiopoietin 1; RV: right ventricle; LV: left ventricle; IVS: interventricular septum. Values are means ± SEM of a minimum of four observations (in vitro experiments) or four animals (in vivo experiments) in each group. *P <0.05, **P <0.01, compared with controls, 1-way ANOVA
