Fig. 4

PLCγ1/PLCγ2 double deficiency alters gene expression in CLPs and B cell progenitors. BM from Plcg1 ^+/+ Plcg2 ^+/+, Plcg1 ^−/− Plcg2 ^+/+, Plcg1 ^+/+ Plcg2 ^−/−, or Plcg1 ^−/− Plcg2 ^−/− mice were transplanted into lethally irradiated congenic wild-type CD45.1⁺ mice. Six to eight weeks after transplantation, CLPs (CD45.2⁺Lin⁻IL-7R⁺) and B cell progenitors (CD45.2⁺B220⁺IgM⁻IL-7R⁺) were sorted from the BM transplantation recipients and subjected to RNA-seq analysis. a Differentially expressed genes in Plcg1 ^−/− Plcg2 ^−/− (upper), Plcg1 ^−/− Plcg2 ^+/+ (middle), or Plcg1 ^+/+ Plcg2 ^−/− (lower) relative to Plcg1 ^+/+ Plcg2 ^+/+ CLPs. Differentially expressed genes with log2 fold-change ≥0.5 and FDR ≤0.05 are shown in red. b Heat maps of differentially expressed genes related to T and B lymphocytes in Plcg1 ^−/− Plcg2 ^−/− relative to Plcg1 ^+/+ Plcg2 ^+/+ CLPs. c Differentially expressed genes in Plcg1 ^−/− Plcg2 ^−/− (upper), Plcg1 ^−/− Plcg2 ^+/+ (middle), or Plcg1 ^+/+ Plcg2 ^−/− (lower) relative to Plcg1 ^+/+ Plcg2 ^+/+ B cell progenitors. Differentially expressed genes with log2 fold-change ≥0.5 and FDR ≤0.05 are shown in red. Heat maps of differentially expressed genes related to B cell lineage (d), IL-7 signaling (e), cell cycle (f), and cell apoptosis (g) in Plcg1 ^−/− Plcg2 ^−/− relative to Plcg1 ^+/+ Plcg2 ^+/+ B cell progenitors are shown. Data shown are obtained from three mice of each genotype