Fig. 5
From: Emergent patterns of collective cell migration under tubular confinement
PIV analysis of MDCK cell tubes in microtubes of different diameters. a Direction of velocity fields showing backward and forward motion in 25 μm tubes (left), highly directed forward motion in 100 μm tubes (middle) and multiple leading edges (white arrows) in 250 μm tubes (right). Scale bars: 75 μm. b Longitudinal and circumferential correlation length of velocity vectors (n = 14 from five independent experiments). c Order parameter (S v = < cos(ϕ) > ) of the velocity vectors in MDCK TCSs (n = 6 from three independent experiments). d Kymograph of the average velocity parallel to the longitudinal axis of different microtube (y-direction in a, from left to right: 25, 100, and 250 μm). e Kymograph of the average velocity along the tubular circumferential of different microtube (x-direction in a, from left to right: 25, 100, and 250 µm). The plots represent the mean ± s.e.m. For each condition, t-test has been performed between each microtube diameter and 25 μm, unless otherwise indicated by lines, *P < 0.05, **P < 0.01, ***P < 0.001, NS non-significant
