Fig. 1
Cell cycle and polarity cues regulate Myosin’s dynamic relocalization. a Representative image sequence showing a wild-type neuroblast expressing Sqh::GFP (Myosin, white; top row, green; bottom row) and the DNA marker His2A::mRFP (red; bottom row). Cortical Myosin intensity was measured at the apical (dark green dashed box) and basal cortex (light green dashed box) throughout mitosis and plotted in b. Chromatid segregation starts at “0 s”. c Mean apical and basal Myosin clearing time and standard deviation in relation to chromatid segregation. The blue and orange numbers represent the mean value. d Kymographs showing Myosin intensity at the apical (blue boxes), lateral (green boxes) and basal neuroblast cortex (orange boxes) for one representative wild-type neuroblast. Kymographs were generated from high temporal resolution time-lapse movies (2 s acquisition time). The graph shows Myosin intensity at the apical (blue plot), lateral (green plot) and basal cortex (orange plot). Apical e basal f Myosin clearing time after nuclear envelope breakdown (NEB) in control and Flavopiridol-treated neuroblasts. g Equatorial Myosin accumulation time after NEB. h Scatter plot showing the apical and basal Myosin clearing time in relation to anaphase onset, for wild-type and dlg;;pins mutant neuroblasts. For this and all subsequent figures: since polarity is lost in dlg;;pins mutants, we refer to the cortex clearing slightly earlier or associated with the slightly bigger cell as “apical”, whereas the other cortex is referred to as “basal”. Center values and error bars represent the mean and standard deviation (s.d), respectively. Asterisks denote statistical significance, derived from unpaired t-tests: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****P ≤ 0.0001. Each measured cell (n) is represented with a dot in the scatter plots. For other graphs, the number of measured cells is indicated in the corresponding panels. For each experiment, the data was collected from at least 3 independent experiments. For each independent experiment, at least 5 larvae were dissected. Time: seconds (s). Scale bar: 5 µm. Time scale bar (open white box) in (d): 20 s. n.s. not significant
