Fig. 4

The centralspindlin complex induces basolateral Myosin enrichment, necessary for basal Myosin relocalization. a Representative wild-type neuroblasts expressing Tum::Venus (white; middle row, green; overlay), and stained for alpha-Tubulin (αTub; red) and phospho-Histone3 (PH3; white in first and third row). Higher magnification images correspond to regions highlighted with yellow and blue squares. b Representative image sequence of a wild-type neuroblast expressing Myosin (white; top row, red; overlay in third row) and Tumbleweed (white; second row, green; overlay in third row). Higher magnification pictures were taken from the regions highlighted with white dashed boxes and shown as a merge (third row). Myosin (red) and Tumbleweed (green) intensity plots, obtained from the apical to the basal cortex, are shown for each time point. Representative image sequences of third instar neuroblasts expressing Sqh::GFP (Myosin; green) and Cherry::Jupiter (MTs; purple) for (c) wild type, (d) colcemid-treated rod mutant, (e) Tum or (f) Pebble depleted neuroblasts. Myosin intensity was measured from the apical to the basal neuroblast cortex at three different time points. For all conditions, the mean intensity, derived from 5 cells and standard deviation is plotted. The data were collected from at least 3 independent experiments. The star corresponds to the lateral Myosin enrichment detected in early anaphase in wild-type neuroblasts. Scale bars: 5 µm