Fig. 2
From: Acetylated histone variant H2A.Z is involved in the activation of neo-enhancers in prostate cancer
H2A.Zac occupancy at genomic regions in PrEC and LNCaP. a Genomic Association Test (GAT) of H2A.Zac ChIP-seq and ChromHMM regions in LNCaP and PrEC cells. A total number of 42,538 H2A.Zac intersecting peaks from biological replicates were detected in LNCaP and 31,479 H2A.Zac peaks in PrEC. Pie charts (upper panel) representing the percentage of marked H2A.Zac peaks falling in each ChromHMM state. Observed vs. expected fold enrichment graphs (lower panel), * p-value < 0.0001 of significant enrichment. The line indicates the threshold of the significant enrichment. b Ngs plots of the average signal (read count per million mapped reads) of H2A.Zac ChIP seq in LNCaP (red) and PrEC (green) cells for each genomic region of significant enrichment. The plots were centered at the transcriptional start site (TSS) in the case of active and bivalent promoters and at the midpoints of DNase I hypersensitive sites sequencing (DNAseI) peaks in the case of active or poised enhancers. c Heatmaps showing H2A.Zac ChIP-seq signal (ordered by H2A.Zac signal intensity from top to bottom) across all the assigned active and poised enhancers, according to ChromHMM. H2A.Zac was centered using DNAseI midpoint. Dashed line separates enhancers as “with H2A.Zac” or “ without H2A.Zac”. Scale bar shows the colorkey of the intensity of H2A.Zac ChIP average signal (read count per million mapped reads, RPM)
