Fig. 3
Characterisation of the PHAX-CBC interaction. a ITC data and curve fit to derive the affinity of PHAX constructs for CBC. CBC or m7GTP-CBC in the sample cell was titrated by PHAX. The data were plotted as described in the caption to Fig. 1c. b Identification of cross-linked lysines between CBC and PHAX by XL-MS. The CBC–PHAX complex was purified by SEC and cross-linked using DSS. After digestion and purification by SEC the cross-linked peptides were identified using mass spectrometry. c Location of the identified PHAX cross-linked lysines (green) on the surface of CBC (CBP80 purple, CBP20 pink, PDB: 1H2V)
