Fig. 5
Crystal structure of the CBC–ARS2827–871 complex. a Crystal structure of m7GTP-CBC-ARS2827–871. CBC is depicted as a semi-transparent surface with underlying secondary structure as ribbons with CBP20 and CBP80 in pink and purple, respectively. ARS2852–871 is shown as yellow sticks and m7GTP as cyan spheres. b Zoomed view of the CBC–ARS2 interface highlighting key ARS2 interacting residues Arg854 (left), Tyr859 (middle), and Phe871 (right). Putative hydrogen bonds are indicated with black dotted lines. c ITC data of ARS2827–871 mutants and CBC and ARS2827–871 and CBCmut in the presence of m7GTP. m7GTP-CBC in the sample cell was titrated by ARS2827–871. The data were presented as described in the caption to Fig. 1c. K D values represent the average from at least two independent experiments (Table 1)
