Fig. 3 | Nature Communications

Fig. 3

From: Two missense mutations in KCNQ1 cause pituitary hormone deficiency and maternally inherited gingival fibromatosis

Fig. 3

KCNQ1 is expressed in cells of the mouse hypothalamic–pituitary growth hormone axis. a Immunofluorescence staining against KCNQ1 (red) and Growth Hormone (GH, green) reveals membranous expression of KCNQ1 in a subset of GH-expressing cells of the postnatal pituitary at day 17 (P17, yellow arrowhead) as well as expression in additional pituitary cell types (white arrowhead) and surrounding blood vessels (arrows). b Sensitive RNAscope in situ hybridization detects Kcnq1 mRNA (aqua) in Ghrh-expressing neurons of the developing hypothalamus (red) at 17.5 days post coitum (dpc), and confirms the expression in Gh-expressing somatotropes (red) at P17. Kcnq1 is expressed in additional endocrine cells types such as Lhb-expressing gonadotropes (red). Examples of double-positive cells are noted by yellow arrowheads. c Kcnq1 mRNA is detected in cells surrounding blood vessels of the developing anterior pituitary at 15.5 dpc. Scalebars indicate 100 μm in low magnification images and 20 μm in high magnification images. AL anterior lobe, BV blood vessel, Ht hypothalamus, IL intermediate lobe, 3v third ventricle

Back to article page