Fig. 4

Cysteine palmitoylation is restricted by palmitoyl-acyl transferases (PAT) accessibility. PATs are restricted to lateral diffusion for its catalytic DHHC domain to interact with substrates. The membrane orientation of Cld3 prevents PATs to access palmitoylation sites located on helix 3, whereas palmitoylation sites on helix 1, 2, and 4 are accessible