Fig. 1

Decreased bone marrow and splenic NK cells in Stat5 double knockin (DKI) mice. a Total bone marrow cell numbers from 8 WT (open bar) and 8 Stat5 DKI (filled bar) mice after removal of red blood cells. b Representative flow cytometric data of total bone marrow NK cells (lin(CD19, CD3, TCRβ, CD4, CD8, Ter119)⁻CD122⁺) (upper panels) and progenitor (NK1.1⁻DX5⁻), immature (NK1.1⁺DX5⁻), and mature (NK1.1⁺DX5⁺) NK cells (lower panels) in WT and Stat5 DKI (DKI) bone marrow. The numbers are the percentage of gated populations. c Total bone marrow NK (Total), NK progenitor (NKp), immature NK (iNK) and mature NK (mNK) cell numbers from 8 WT (open bars) or 8 DKI (filled bars) mice. d Representative flow cytometric data of total bone marrow NK cells from WT and Stat5 DKI mice gated as lin^-CD122⁺NK1.1⁺DX5⁺ (lower panels of b) and further characterised based on CD11b and CD27 staining. e Numbers of total bone marrow NK cells (Total) and CD11b⁻CD27⁺, CD11b⁺CD27⁺, and CD11⁺CD27^low populations from 8 WT (open bars) and 8 Stat5 DKI (filled bars) mice. f Total splenocyte numbers from 8 WT (open bar) and 8 Stat5 DKI (filled bar) mice, after removing red blood cells. g Representative flow cytometric data of total splenic NK cells from WT and Stat5 DKI mice gated as CD3⁻CD122⁺NK1.1⁺ (upper panels) and further characterised based on CD11b and CD27 staining (lower panels). h Number of total splenic NK cells (Total), CD11b⁻CD27⁺, CD11b⁺CD27⁺, and CD11⁺CD27^low populations from 8 WT (open bars) and 8 DKI (filled bars) mice. Error bars in c, e and h are means ± SEM and statistical analyses were performed by grouped multiple t-test using Prism 7.0b