Fig. 3
From: Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells
Engineered sphere cells exhibit gene expression patterns of proximal tubule epithelial cells. a Overview of experimental procedure to identify the gene expression profile of engineered shRNA-Tsc2 + Cdkn2a AML-forming sphere cells. b Venn diagram showing the overlap of gene expression signature of engineered spheres (green) with distinct mRNA signatures of S1 and S3 segments of proximal tubules (blue) or a pooled mRNA signature of DCT (distal convoluted tubule), TAL (thick ascending limb of the Loop of Henle) and CD (collecting duct) (red). c Real time PCR analysis of the indicated genes in cultures of control kidney cells (total pKC population), total sphere populations, sphere cultures of SC2, SC3, SC4 and SC6 and AML-like allograft tumours.Graph depicts mean ± s.d. Student’s t test, n = 3. ∗∗P < 0.01; ∗∗∗P < 0.001. d Positive immunofluorescence stainings of spheres using antibodies against NAPI2A, CATHEPSIN K, pan-CD133, MiTF, PMEL(HMB45) and MART-1. e Positive immunofluorescence and immunohistochemical stainings of AML-like allograft tumours using antibodies against NAPI2A, RCC, E-CADHERIN, CD10, CD133 and pan-CYTOKERATIN. Scale bars: 50 μm
