Fig. 5 | Nature Communications

Fig. 5

From: Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity

Fig. 5

Introduction of mutant Kras variants into muscle cells using AAV/Cas9-mediated HDR induces invasive sarcoma. a Schematic of intramuscular administration of AAV-Kras HDR/sgKras/Cre (1.6 × 1011 vector genomes per mouse) into the gastrocnemii of PT;H11 LSL-Cas9 mice to induce sarcomas. b, c Histology of stereotypical sarcoma (b) and invasive sarcoma (c) initiated by intramuscular administration of AAV-Kras HDR/sgKras/Cre into the gastrocnemii of PT;H11 LSL-Cas9 mice. Scale bars = 75 µm. d Sarcoma incidence in PT;H11 LSL-Cas9 mice 3–7 months after intramuscular administration of AAV-Kras HDR/sgKras/Cre. Incidence represents the number of mice that developed sarcomas out of the total number of mice injected. e HDR-generated oncogenic Kras alleles in sarcomas. Number of tumors with each allele is indicated. Alleles not identified in any sarcomas are not shown

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