Fig. 1
From: Insights into the structure and assembly of a bacterial cellulose secretion system
E. coli-like cellulose secretion systems—components and macrocomplex detection. a E. coli bcs operon organization (top) and examples of organisms sharing E. coli-like cellulose secretion system conservation. b Predicted localization and function of the encoded Bcs components. Domain architectures for all Bcs proteins are detailed in Supplementary Fig. 1. c Chromosomal epitope-tagging of the BcsQ and BcsA subunits and functional validation of the recombinant proteins. The sequence of the respective N-terminal and C-terminal epitope tags is shown on the left; calcofluor-binding and fluorescence of the recombinant E. coli 1094 strains is shown on the right. d Thumbnail summary of co-purifying Bcs partners as determined by anti-FLAG affinity purification and mass-spectrometry analyses
