Fig. 5
From: Chromosome segregation occurs by microtubule pushing in oocytes
CLS-2CLASP microtubule polymerase activity is required for meiotic chromosome segregation. a BUB-1::mCherry (top row), CLS-2CLASP::GFP (middle row), and merged (bottom row) localization during anaphase I. Asterisks denote the approximate centroid locations of the segregating chromosome masses. b GFP-tagged CLS-2CLASP and CLS-2CLASP 3A localization during anaphase I in the indicated conditions. c Still images from live imaging of fertilized oocytes expressing GFP-tagged α-tubulin and mCherry-tagged histone H2B in the indicated conditions. Quantifications of the percentage of observed bipolar spindles, chromosome segregation events, and polar body extrusion in meiosis I is shown on the right of each still sequence. Timings are indicated in the bottom left corner of each frame. Scale bars, 5 µm
