Fig. 4 | Nature Communications

Fig. 4

From: The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding

Fig. 4

Mutational analysis of E9 insert 3 regarding A20 C-ter binding. a, c, e Crystal structure of E9 insert 3 (gray) with mutated residues highlighted. The corresponding sequence of the α-helix of insert 3 (black letters) is shown with mutated amino acids depicted as colored letters. b, d, f Each E9 mutant was mixed with WT A20 C-ter prior to analysis by size exclusion chromatography. A 4–20% SDS-PAGE analysis of the eluted fractions for each of the runs is presented

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