Fig. 6
From: Membrane curvature induced by proximity of anionic phospholipids can initiate endocytosis
Acute removal of cholesterol forms PtdSer-rich tubules. a Confocal images of following mβCD treatment of HeLa cells co-transfected with mRFP-LactC2 and GFP-K-Ras tail. Lower left insets: magnification of the areas indicated by a dashed square. Lower right insets: magnification of areas indicated by a lined square. b Quantitation of the distribution of GFP-K-Ras tail between the plasma membrane and the cytoplasm 20 min after the addition of mβCD. Data are means ± s.e.m., n = 14. c, e mβCD treatment of HeLa cells co-transfected with mRFP-LactC2 and either the PtdIns4P probe GFP-P4M-SidMx2 or the PtdIns4,5P2 probe GFP-PLCδ-PH. Insets: magnification of the areas indicated by a square. d, f Quantitation of the distribution of GFP-P4M-SidMx2 or GFP-PLCδ-PH between the plasma membrane and the cytoplasm 20 min after the addition of mβCD.Values represent the means ± s.e.m., n = 11. g Confocal images of control, DDPS and acute mβCD-treated HeLa cells co-expressing Synaptojanin2-mCherry and Endophilin2-GFP. NS = not significantly different, *p < 0.05, **p < 0.01 and ***p < 0.005; as determined using a two-tailed t-test. Scale bar = 10 µm
