Fig. 2

uc.339 promotes cancer cell growth and migration. a Cell viability assay in A549 and LoVo cells infected with a lentiviral vector overexpressing uc.339 (LV-uc.339) or its empty vector counterpart (LV-E) and detected after 72 h. Data are presented as mean ± s.d. of experiments conducted in triplicate and normalized to LV-E. *P < 0.05. b Representative image of a scratch migration assay in A549 LV-E and A549 LV-uc.339 cells at 0, 8, and 24 h. Scale bar = 200 μm. c Quantification of the percentage of the wound area of the scratch migration assay shown in b and represented as mean ± s.d. of experiments conducted in triplicate. **P < 0.01. d Cell viability assay in A549, H460, H1299, and LoVo cells transfected with two different si-uc.339 or si-SCR for 72 h. Data are presented as mean ± s.d. of experiments conducted in triplicate and normalized to si-SCR. *P < 0.05. e Cell-cycle analysis (shown as the percentage of cells in G₀/G₁ or G₂/M or S-phase of the cell cycle) conducted by cytofluorimetry with propidium iodide staining in A549, H460, H1299, and LoVo cells transfected with si-uc.339(1) or si-SCR for 72 h. Data are presented as mean ± s.d. of experiments conducted in triplicate. **P < 0.01. ***P < 0.001. f Immunoblotting for cleaved PARP and Vinculin in A549, H460, H1299, and LoVo cells transfected with si-uc.339(1) or si-SCR for 72 h. The numbers above the bands represent the quantification of the band intensity, calculated with Quantity One software and normalized to Vinculin and si-SCR