Fig. 4
From: Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology
dCK binding to protein kinase inhibitors and substrates determined by ITC. Representative ITC titrations for dCK with two protein kinase inhibitors (masitinib a, imatinib b) and four pyrimidine substrates (2′dC c, gemcitabine d, decitabine e, cytarabine f) at 25 °C as a function of the molar ratio of ligand to protein. The upper panel shows the ITC raw data and the lower panel presents the integrated heat of each injection. Binding isotherms were fitted to the raw data using one-site or two-sites binding models as indicated to determine the K D. For substrates, there was one molecule par protein, but for masitinib and imatinib, two binding sites were obtained. Each experiment was performed three times and data are presented as the mean ± s.d
