Fig. 2

Lack of compartmentalisation in the ΔftsZ mutant. a–c Panels show FM4-64 membrane-stained wild-type cells grown in GYM media in a home-made microfluidic device. Panels b and c are equivalent, but with lower brightness in the latter. White arrow indicates the cell division site. Yellow asterisks show possible membrane accumulations. Scale bars = 5 µm. d–g Panels show bright field (d, e) and FM4-64 stained membrane (f, g) of the ΔftsZ mutant grown in GYM in a home-made microfluidic device. Scale bars = 5 µm. h, i growth of wild-type (h) and ΔftsZ mutant (i) cells in the CellASIC ONIX microfluidic chamber in TSB media with constant lysozyme flow up to 1000 µg ml⁻¹. White arrows indicate the presumed presence of a division site. Scale bar = 5 µm