Fig. 4

Cell regrowth following fragmentation. a DivIVA tip localisation in wild type (left panel) and ΔftsZ mutant (right panel). b Representative examples of the types of cell regrowth of fragments in the ΔftsZ mutant from both ends (top), multiple/new sites (middle) and from an existing pole and/or bulge (bottom). All spores and fragments were grown in the CellASIC ONIX microfluidic chamber with TSB medium, using a 500 ms exposure for acquiring the mCherry signal. White arrows indicate the sites of DivIVA-mCherry localisation. Scale bars = 10 µm in a and 5 µm in b