Fig. 6
NK cell HIF-1α deficiency facilitates tumour cell intravasation and pulmonary metastasis. a The quantification of the metastatic foci and metastatic area in LLC tumour-bearing mice determined by H&E staining on 10 μm lung paraffin serial sections at day 14 post tumour injection. b Tumour volume analysis of B16F10 melanoma isografts implanted in WT and HIF-1α KO mice. c Quantitative analysis of CD31-positive endothelial cells and pericyte coverage as assessed by α-SMA/CD31 co-localisation at endpoint, day 14. d Quantitative analysis of hypoxic tumour areas with the specific marker GLUT1 at endpoint, day 14. e Quantitative analysis of caspase-3-positive areas at endpoint, day 14. f Determination of levels of sVEGFR1 and VEGF protein in B16F10 melanoma isografts implanted in WT and HIF-1α KO mice by ELISA at endpoint, day 18. g Gene expression analysis of the melanoma-specific gene S100B in peripheral blood (day 14 post tumour inoculation) and h lungs from melanoma-bearing animals (at endpoint, day 18 post tumour inoculation) (n = 10 for WT group; n = 6 for HIF-1α KO group). Statistical significance was determined by an unpaired Student’s t-test or one-way analysis of variance, where appropriate. Bars represent mean values; error bars indicate the s.e.m. Statistical significance is indicated as *P < 0.05, **P < 0.01, and ***P < 0.001
