Fig. 5
From: STIM1 promotes migration, phagosomal maturation and antigen cross-presentation in dendritic cells
Stim1 ablation does not affect BMDC differentiation, maturation, or phagocytic rate. a BMDCs express CD11c, CD11b, low levels of F4/80 and MHC-I to similar extents regardless of STIM1 expression. N = 8/5/5 for CD11c/CD11b/F4/80. Values of cellular autofluorescence (−Ab) are shown for comparison. The gating strategy is shown in Supplementary Fig. 5a. b Expression of CD40, CD80, CD86 and MHC-II in immature cells or in cells matured with either CpG or LPS was similar in cells expressing or not STIM1. The full gating strategy is shown in Supplementary Fig. 2c. N = 7/5/3 for CTR/CpG/LPS for CD40/CD80, 8/5/7 for CD86 and 8/5/5 for MHC-II. c Phagocytosis of YG- Fluoresbrite-OVA-coated beads (OVAb) was decreased by loading cells with 40 μM BAPTA but not by exposing cells to the non-specific SOCE blocker LaCl3 (50 μM), and was similar in immature and mature STIM1-deficient cells as compared to cells from wild-type littermates. N = 4/5/3/3/3 for 30 min/4 h/24 h/BAPTA/La3+. The full gating strategy is shown in Supplementary Fig. 2c. d The absence of phagocytic defect in STIM1-deficient cells was confirmed by quantifying phagocytosis by microscopy. Phagocytic targets added at 20:1 targets:cells. N = 3. MFI = mean fluorescence intensity. Black bar = 3 μm. Error bars are means + SEM, *p < 0.5 using a Student’s t-test
