Fig. 1

PU.1 and SpiB regulate follicular B cell numbers. a Flow cytometric analysis of splenocytes from control (Cd23 ^T/+), PU.1 cKO (Spi1 ^fl/fl Cd23 ^T/+), SpiB KO (Spi1 ^fl/fl Cd23 ^+/+ Spib ^−/−) and PU.1 SpiB DKO (Spi1 ^fl/fl Cd23 ^T/+ Spib ^−/−) mice showing the abbreviated gating strategy for the detection of follicular (Fo) B cells (B220⁺ IgD⁺ IgM^int) and MZ (B220⁺ IgM⁺ IgD^low CD21⁺) B cells. As the loss of PU.1 and SpiB leads to the downregulation of CD23 expression, follicular B cell numbers were based on the gate for B220⁺ IgD⁺ IgM^int. The full gating strategy is shown in Supplementary Fig. 8A. Data are representative of 7–10 mice per genotype. b Absolute cell numbers are graphed as the mean ± s.d. P-values compare the indicated samples (two tailed t-test). Each circle represents the results from an individual mouse. *P < 0.05, **P < 0.01, ****P < 0.0001