Fig. 2

Trf2 is required for the male-specific neurite formation in mAL neurons. a–d mAL neuroblast clones without (a, c), or with (b, d) expression of Trf2 RNAi in male (a, b), or female (c, d) flies. e, f Single-cell clones with (e) or without (f) the ipsilateral neurite in a control male (e) and a male with Trf2 knockdown (f). g Quantitative comparisons of the frequency of obtaining single-cell clones with and without the ipsilateral neurite between wild-type and Trf2 knockdown mAL neurons in males. Statistical differences were evaluated by the χ 2-test (g). *P < 0.05. Scale bar: 50 µm (a)