Fig. 4

TRF2-S and FruBM regulate transcription from the robo1 promoter. a Schematic representation of the exon–intron organization for two robo1 transcripts (robo1-RA and robo1-RC) and the robo1 reporter construct. b A luciferase reporter fused to the robo1 promoter fragment of 1.7 kb exhibited an elevated transcription in the presence of TRF2-S alone and a reduced transcription in the presence of FruBM alone. c A lower dose of FruBM, which by itself had no effect on the reporter activity, was able to exert a strong repressor effect in the presence of TRF2-S. All experiments were conducted as triplicate and the mean ± SEM of relative luciferase activities are shown. Statistical differences were evaluated by one-way ANOVA followed by Bonferroni’s multiple comparison test. ***P < 0.001, **P < 0.01, *P < 0.05; ns not significant