Fig. 3

Identification of Grs for LOB, BER, and DEN detection in S-b sensilla. a–c Screening to identify the Grs required for the responses in S-b sensilla to LOB a, BER b, and DEN c. 1 mM of each chemical was used. n = 8–31. ANOVAs with Tukey or Dunnett T3 post hoc tests or Kruskal−Wallis tests with Mann−Whitney U post hoc tests as appropriate. The asterisks indicate statistically significant differences from wild-type flies. d Representative traces from wild-type and Gr22e ¹ S5 sensilla upon application of LOB, BER, and DEN. e Mean responses of the indicated sensilla from wild-type and Gr22e ¹ flies to 1 mM stimulations with the indicated bitter substances except 5 mM CAF. n = 6–30. Unpaired Student’s t-tests or Mann−Whitney U-tests as appropriate. f Mean responses of Gr32a, Gr66a, and Gr22e-expressing L-type sensilla induced by 1 mM stimulations with the indicated chemicals except 100 mM SUC and 5 mM CAF. n = 7–14. Unpaired Student’s t-tests or Mann−Whitney U-tests as appropriate. All data are presented as means ± S.E.M. *p < 0.05, **p < 0.01. Complete genotypes are listed in Supplementary Table 6