Fig. 4 | Nature Communications

Fig. 4

From: Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity

Fig. 4The alternative text for this image may have been generated using AI.

The gl precursor of the PGT128/130 families binds the lead NIT82B glycoconjugate. Binding of the predicted gl antibody was determined by flow cytometry using a biotinylated version of the lead conjugate NIT82B (designated NIT150b). Shown is the binding of the gl precursor (left) and of the affinity-matured PGT128 antibody (right), as a control, to NIT150b at 1 µM. Bound antigen was detected with PE-conjugated streptavidin and antigen binding gated on the live, antibody-expressing cell populations, which were detected with an APC-conjugated anti-human F(ab‘)2-specific antibody. The greyed histograms represent glycoconjugate binding to cells transfected with the HIV bnAb VRC01, which is specific for the CD4-binding site of HIV Env. The results are from a single experiment

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