Fig. 4

Cell viability and cell cycle profiles upon single NUDIX depletion. a Survival of CCD841, A549, MCF7, and SW480 cells upon single depletion of the NUDIX enzymes using a pool of four siRNA sequences. The survival was measured by resazurin and normalised to the non-targeting siRNA control. b Cell cycle profiles upon single NUDIX knockdown in CCD841, A549, MCF7, and SW480 cells. The histograms were obtained by measuring the integrated intensity of the DNA counterstained with Hoechst and the signal was then processed using PopulationProfiler as described in⁴⁶