Fig. 1
From: Epigenetic targeting of bromodomain protein BRD4 counteracts cancer cachexia and prolongs survival
Response to JQ1 in cancer cell lines used to study cancer cachexia. a Viability rates of JQ1-treated cells, calculated by measuring the viable cell number after 72 h in culture. Results are normalized to the proliferation of vehicle/DMSO-treated cells, set to 100% (n = 3). Statistical analysis was performed by using one-way ANOVA followed by Tukey’s post hoc test. Data represent means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 vs. DMSO. b Ten days after B16-tumor cells inoculation, mice were treated either with vehicle or (+)-JQ1 (20 mg/kg/day) for 10 days. Tumor weight after necropsy is shown. Animals per group: n = 7. Data represent means ± SD. Statistical analysis was performed by using Student’s unpaired t-test. ***p < 0.001 indicates statistical significance vs. B16 + veh. c Tumor weight at the day of sacrifice (day 12) in C26-tumor-bearing mice (animals per group: n = 10). Data represent means ± SD. Statistical analysis was performed by using one-way ANOVA followed by Tukey’s post hoc test. d c-Myc and cleaved-Caspase-3 levels were quantified by immunoblots using tumor whole extracts (animals per group: n = 4)
