Fig. 7
Immuno-PET imaging to demonstrate the induction of the systemic immune response by OX/IND-MSNP administration to animals carrying orthotopic KPC tumors. a Animals with established orthotopic tumors (n = 3/group) were IV injected with saline, OX/LB-MSNP (5 mg/kg OX), and OX/IND-MSNP (5 mg/kg OX and 50 mg/kg IND on days 10, 14, 18, and 22 post KPC cell implantation into the pancreas. At day 26, 100 µL doses containing 1.07–2.33 MBq (29–63 µCi, 2.3–5.3 µCi/µg)89Zr radiolabeled cDb in saline was IV injected to the same animals. 20 h later, microPET and CT scans were acquired by a G8 PET/CT scanner (Sofie Biosciences). Coronal (left panel) and transverse views (right panel) were acquired and analyzed by AMIDE software. OX/IND-MSNP-treated mice showed significantly increased radioactivity in the tumor, spleen, and TDLN, corresponding to the induction and infiltration of CD8+ T cells. b To evaluate the CD8+ signal at the tumor site, the operator-defined ROIs were used to demonstrate a 6.2- and 7.5-fold increase in the signal intensity in the tumor interior and periphery, respectively, during OX/IND-MSNP compared to saline treatment. The results are expressed as mean ± SEM. *p < 0.05; **p < 0.01, (ANOVA)
