Fig. 1
From: The activity of TRAF RING homo- and heterodimers is regulated by zinc finger 1
Structural characterisation of TRAF6 in complex with a Ubc13~Ub conjugate. a Schematic showing the domains of TRAF6, with the RZ3 and RZ1 constructs indicated below. ZF: zinc finger, CC: coiled-coil. b Multi-turnover activity assay comparing H. sapiens and D. rerio RZ3 TRAF6. Each form of TRAF6 RZ3 was incubated with E1, Ubc13-Uev1A and ubiquitin at 37 oC for the indicated times. c Analytical size exclusion profile of 250 µM DrRZ3 and the isopeptide-linked Ubc13–ubiquitin conjugate (Ubc13~Ub) alone, or a mixture of the two were separated using a Superdex 200 10/300 column. Equivalent fractions from each run were resolved by 16% SDS-PAGE. d Crystal structure of the complex of DrRZ3 and Ubc13~Ub showing the asymmetric unit. The two TRAF6 monomers are in shades of green, ubiquitin is yellow and Ubc13 is blue. The disordered ZF2 and ZF3 are indicated in grey in the schematic
